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ngctm medium pressure liquid chromatography system questtm 10  (Bio-Rad)
96
Bio-Rad ngctm medium pressure liquid chromatography system questtm 10
Ngctm Medium Pressure Liquid Chromatography System Questtm 10, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ngctm+medium+pressure+liquid+chromatography+system+questtm+10/pmc10549239__CB___004___D3CB00107E___s001-37-17-25?v=Bio-Rad
Average 96 stars, based on 1 article reviews
ngctm medium pressure liquid chromatography system questtm 10 - by Bioz Stars, 2026-07
96/100 stars
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chromatography system  (Bio-Rad)
96
Bio-Rad chromatography system
Fig. 3. REST-FL purification. (a) Analysis of Im7 and His-trap purification steps using 10 % SDS-PAGE. M – protein molecular weight marker; Sol – soluble fraction of crude extract; FT – flow-through fraction; Elu – collected elution fraction from Im7 column after cleavage. His-trap column elution samples are denoted with peak number and percent of imidazole gradient: H1 (5 % imidazole) contains REST-FL, and H2 (50 % imidazole) contains SrtA protease. (b) His-trap column <t>chromatography</t> traces. His-trap column separated the protease and low molecular weight contaminations from REST-FL. Fraction marks correspond to the fractions analyzed in (a). Fraction H1 contained REST-FL. H2 fraction corresponds to SrtA protease. Imidazole absorbance is projected to the absorbance trace according to the imidazole concentration. Green dashed lines indicate collection ranges.
Chromatography System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ngctm+medium+pressure+liquid+chromatography+system+questtm+10/pm36698979-276-17-25?v=Bio-Rad
Average 96 stars, based on 1 article reviews
chromatography system - by Bioz Stars, 2026-07
96/100 stars
  Buy from Supplier

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Fig. 3. REST-FL purification. (a) Analysis of Im7 and His-trap purification steps using 10 % SDS-PAGE. M – protein molecular weight marker; Sol – soluble fraction of crude extract; FT – flow-through fraction; Elu – collected elution fraction from Im7 column after cleavage. His-trap column elution samples are denoted with peak number and percent of imidazole gradient: H1 (5 % imidazole) contains REST-FL, and H2 (50 % imidazole) contains SrtA protease. (b) His-trap column chromatography traces. His-trap column separated the protease and low molecular weight contaminations from REST-FL. Fraction marks correspond to the fractions analyzed in (a). Fraction H1 contained REST-FL. H2 fraction corresponds to SrtA protease. Imidazole absorbance is projected to the absorbance trace according to the imidazole concentration. Green dashed lines indicate collection ranges.

Journal: Computational and structural biotechnology journal

Article Title: Electron microscopy reveals toroidal shape of master neuronal cell differentiator REST - RE1-silencing transcription factor.

doi: 10.1016/j.csbj.2022.12.026

Figure Lengend Snippet: Fig. 3. REST-FL purification. (a) Analysis of Im7 and His-trap purification steps using 10 % SDS-PAGE. M – protein molecular weight marker; Sol – soluble fraction of crude extract; FT – flow-through fraction; Elu – collected elution fraction from Im7 column after cleavage. His-trap column elution samples are denoted with peak number and percent of imidazole gradient: H1 (5 % imidazole) contains REST-FL, and H2 (50 % imidazole) contains SrtA protease. (b) His-trap column chromatography traces. His-trap column separated the protease and low molecular weight contaminations from REST-FL. Fraction marks correspond to the fractions analyzed in (a). Fraction H1 contained REST-FL. H2 fraction corresponds to SrtA protease. Imidazole absorbance is projected to the absorbance trace according to the imidazole concentration. Green dashed lines indicate collection ranges.

Article Snippet: REST-N62 was purified in three-step chromatography, carried out in a cold room at 4 °C with a chromatography system (NGCTM Chromatography System QuestTM 10 Plus, Bio-Rad).

Techniques: Purification, SDS Page, Molecular Weight, Marker, Column Chromatography, Concentration Assay